![]() The principle and details of analysis methods by SLS measurement will be described in this chapter. To explain it more simply, small particles can be analyzed by Debye plots, while large particles are analyzed using Zimm plots. Analysis methods are classified as Debye plots and Zimm plots. Additionally, information of the shape and size of particles can also be obtained. ![]() Various information can be obtained from SLS, for example, the molecular weight, the inertial radius, the second virial coefficient, and the aggregation number of colloidal particles in the solution. Dynamic light scattering (DLS), which is also known as photon correlation spectroscopy ( PCS) or quasi-elastic light scattering (QLS), is a spectroscopy method used in the fields of chemistry, biochemistry, and physics to determine the size distribution of particles (polymers, proteins, colloids, etc.) in solution. The equation is given as: I() I 0 (R 6 / r 2) (1 + cos 2 ) (-4) In this equation: I() represents the intensity of scattered light at angle. DLS is performed by interpreting temporal intensity fluctuations of laser light which is scattered by a dispersion of freely diffusing EVs. The Rayleigh scattering equation quantifies the intensity of scattered light as a function of the scattering angle, the size of the scattering particles, and the wavelength of the incident light. Application of functions specific to nano- and microparticles has become active, and information of particles in the size and shapes mentioned above have important value in academic and industrial research. Dynamic light scattering (DLS), also known as photon correlation spectroscopy (PCS), is a technique for determining the size distribution of vesicles. Static light scattering (SLS) is an excellent method that can nondestructively observe the aggregation number and detailed size information of these particles. Solid microparticles or nanoparticles and molecular aggregates formed by surfactants are dispersed in solutions in order of nanometers to submicrons.
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